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Zymography provided direct evidence of increased proteolytic activity of MMP-2 in lesional skin in comparison with healthy controls. Ninety-one brain tissue sections taken at autopsy from 33 elderly patients (63-100 years old) without progressive multifocal leukoencephalopathy were examined for the presence of JC virus DNA by the polymerase chain reaction and Southern hybridization analysis after DNA extraction. Polylactide and polyglycolic acid-reinforced coralline hydroxy-apatite for the reconstruction of cranial bone defects in the rabbit. This energy dysfunction is induced by anaerobic diversion of glucose metabolism.

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This release could also be mimicked by increasing concentrations of veratridine. To compare the relationships between markers of total and regional adiposity with muscle fat infiltration in type 1 diabetic and type 2 diabetic subjects and their respective nondiabetic controls, and to document these relationships in type 1 diabetic subjects. The clinical heterogeneity and multiple antibody profile may suggest that borderline SLE is a forerunner to SLE rather than a separate entity. The biological effects of simple molecules have traditionally been ascribed to their actions on the lipid portions of biological membranes.

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Three crowns were loaded vertically under the same conditions to serve as the control group. and Swiss travelers who had used no chemoprophylaxis, who had used only chloroquine for prophylaxis, and who had used Fansidar weekly, either alone or in combination with chloroquine. The molecular modeling studies demonstrate that the carboxylate ion of the carbamino adduct at the amino terminus of the beta-chain as well as the carboxylate of carboxymethyl group at the same site are in a geometrical orientation that favors the formation of an intrachain ionic interaction with the epsilon-amino group of Lys-82(beta). Radiological changes show a dose-response relationship for all types, with a median dose for asbestos of 2.8 fibers/ml.

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The characteristics distinguishing this case from others previously presented in the literature include the recurrence, posterior displacement, and age of the patient. Eighteen months previously, he had shown clinical and serologic evidence of mycoplasmal myocarditis.

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Procollagen synthesis was elevated about sixfold on fibroblasts derived from the affected skin. Biochemical properties and subcellular distribution of an N-type calcium channel alpha 1 subunit. This 64-year-old man, left-handed and literate, developed repeated right facial twitching associated with head turning to the right or transient loss of awareness since October 30 1991.

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Transmembrane potentials in Purkinje fibres were studied with standard microelectrodes filled with 3M KCl. At baseline and at study end point, the 2 subgroups demonstrated no statistical differences in scores on PTSD, depression, and anxiety rating scales. We have shown that it is possible to enhance anticoagulant activity and membrane affinity of protein C by selective mutagenesis of the Gla domain.

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Viral genes E6 and E7 of HPV 16 and 18 are regularly expressed in HPV-positive tumours. Hypermetria was found to be increased by artificially increasing the inertia of the moving hand. This histological parameter could be of value to determine the kind of adjuvant chemotherapy after a positive sentinel-node biopsy with only radiotherapy of the axilla and no further axillary lymph-node dissection.

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Recurrent Reye-like syndrome: possible association with Krebs cycle abnormality. Endogenous lectins purified from UV-2237-IP3 murine fibrosarcoma cells by affinity chromatography consisted of two polypeptide species of Mr 14,500 (L-14.5) and Mr 34,000 (L-34). In the next few years progress in developing a sound theoretical foundation will make molecular design a realistic aid to the medicinal chemist and protein engineer.

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Specific cytokines induced during replication of the lentivirus in mononuclear cells are also responsible for directing infected cells from peripheral blood through the vascular endothelium to particular tissues. However, the location of the cell bodies of origin for the serotoninergic projection to the inferior olive in any of these species was unknown.

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Low levels of small molecules which hybridize to probes specific for individual gene cassettes were detected in plasmid DNA isolated from cells containing a plasmid which includes an integron fragment with three gene cassettes aacC1, orfE and aadA2. In this paper, two substantial players in bacterial-associated respiratory disease are assessed as to their respective roles in children and adults and in the developed and developing world.

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The exact function of the GPI anchor has been the subject of much speculation. In this paper we report two interesting cases of acoustic tumors which were diagnosed as sudden deafness at the first examination. These findings indicate that a mucosal memory response can be primed by nonpathogenic strains of Shigella which lack the virulence plasmid. The K(M)s for arginine and glycine were 3.8 and 0.89 mM, respectively, with optimal temperature and pH being 37 degrees C and 9.5, respectively. At this time, kynurenic acid was removed from the culture medium and neurons developed seizure-like activity.

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US with a catheter US probe and sheath allows complete endoscopic examination and preoperative determination of depth of invasion of superficial esophageal carcinoma in a single endoscopic procedure. Moderate IgG and IgM responses occurred when cattle were given 2 vaccinal doses of 0.1 mg or 1.0 mg of whole cells, OMC, or AA. Superposition eyes are generally thought to function ideally when the eye is spherical and with rhabdom tips in the focal plane of the imaging optics of facet lenses and crystalline cones. A turnover study in the male rat of plasma-bound 59Fe, 114Inm and 109Cd with particular reference to the gonad. A Trypanosoma cruzi expression vector has been constructed using sequences derived from the flanking regions of the glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) genes.

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Because the tests are increasingly used in clinical practice and research and because there is much controversy, we reviewed the literature and outlined the potential and possible shortcomings of these procedures. Effects of combined inspiratory muscle and cycle ergometer training on exercise performance in patients with COPD.

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Moreover, the phosphorylated 68-kDa Fos form may be involved in mechanisms underlying SPG proliferation. It can be applied to the detection of all enzymes which are normally detected spectrophotometrically using coupled enzyme assays consuming NAD(P)H. We found a lower incidence of mucocutaneous symptoms and arthritis but an increased incidence of discoid lesions, pleuritis and pericarditis in males at disease onset. Effect of pressure and volume overload on proto-oncogene expression in the isolated working rat heart. We found that AR possesses an intrinsic transcriptional repression activity, and AR interacts directly with SMRT.

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Severe pain at interval IUD insertion: a case-control analysis of patient risk factors. By contrast, all three of our patients had clinical and pathologic evidence of chronic inflammation. According to their functional characterization, genes that were involved in the cytoskeleton and the extracellular matrix were enriched significantly in the up-regulated genes.

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A questionnaire asking about the occurrence of FEIAn in school students was sent to all 145 public junior-high-school nurses in Yokohama. Hyperspectral interferometric microscopy uses a unique combination of optics and algorithm design to extract information. On the other hand, as lower serum and urinary TPA levels were observed after treatment of bladder and prostatic cancer, their determinations were considered to be useful in following up patients with these cancers. The basic helix-loop-helix (bHLH) protein MyoD is a transcription factor that is important for the induction of the myogenic phenotype. These blots demonstrated no gender-based differential expression in rabbit lacrimal or harderian glands.

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Type-specific prevalence of cervical HPV and the presence of cytological abnormalities were determined in a cohort of Lebanese women. Immunologic incrimination of Ancylostoma caninum as a human enteric pathogen.

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Protein kinase C regulates calmodulin expression in NRK cells activated to proliferate from quiescence. Department of Obstetrics and Gynecology, 1st Medical Faculty and General Faculty Hospital, Charles University, Prague, Czech Republic. It is shown that the real distance differs substantially from previous estimates because of large atomic displacements on the surface and at the probe tip.

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Ectopic overexpression of the phytochrome A in several plant species has pleiotropic effects, including substantial dwarfing, increased pigmentation, and delayed leaf senescence. The mode of occurrence of encephalomyocarditis (EMC) virus-induced myocarditis in mice was pathologically and virologically investigated using 2 virus variants (highly diabetogenic EMC-D and non-diabetogenic EMC-B) and 2 mouse strains (diabetes-susceptible BALB/c and diabetes-resistant C57BL/6). We show, using two-hybrid and far-Western assays, that the human member binds to the human yPOP2 homologs, i.e.

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Role of thalamic nuclei in the hypertonia and tremor of Parkinson disease Colour Doppler imaging (CDI) is an ultrasonic method for qualitatively and quantitatively assessing blood flow. This effect could be mediated by a number of organizing factors that we discuss. Distal chick wing bud mesenchyme from stages 19 to 27 embryos has been grown in micromass culture. Development of hearing loss during long-term exposure to occupational noise.

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The commonest cause of a third nerve palsy with pupillary involvement was a posterior communicating artery aneurysm. This review article provides an in-depth discussion of the key elements of drug-eluting stents, focusing on the TAXUS paclitaxel-eluting stent as an example of this new class of product. COX-2 and VEGF messenger RNA expressions were evaluated using Northern blot analysis.

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On the accuracy of homology modeling and sequence alignment methods applied to membrane proteins. T-cell tolerance and autoimmunity in transgenic models of central and peripheral tolerance. Work hardening for warriors: Training military Occupational Therapy professionals in the management of combat stress casualties. Movement-related neurons of the subthalamic nucleus in patients with Parkinson disease. These data suggest that isoproterenol induces a mild inflammatory response within rat submandibular glands that is not observed in normal glands.

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Autocrine flutamide and enzalutamide cost biological effects of glycosyl inositol phosphate produced by reconstituted pig thyroid follicles: role of pertussis toxin sensitive G proteins. Practices related to HR and BP measurement reported by this sample of clinical instructors do not meet the recommendations for physical therapy care described in the Guide. This is a retrospective analysis of transfusion requirements of 43 such patients. The enhanced accessibility to micrococcal nuclease was not restricted to the expressing gene and promoter but involved the entire minichromosome, suggesting that the enhancement of gene expression resulted from changes in the condensation of the entire minichromosomal region by HMG1 protein.

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